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Featured Review
Cell Science Reviews Vol 6 No 3
ISSN 1742-8130 |
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Quantum dot enabled single vesicle imaging - a perfect match between neurobiology & nanotechnology
Qi Zhang 1 & Richard W. Tsien 2
1 Department of Pharmacology, Vanderbilt University, Nashville, Tennessee &
2 George D. Smith Professor of Molecular and Genetic Medicine, Department of Molecular and Cellular Physiology, Stanford University, California, USA.
Received 16th January © Cell Science 2010
In this short review, we focus on the recent application of the quantum dot in neuroscience, particularly in the imaging of single synaptic vesicles. Owing to its superior photo-properties and nanometer size, the quantum dot is ideal for labeling single synaptic vesicles, designated organelles designed to releasing neurotransmitters from presynaptic terminals. Our recent papers have shown its potential to report vesicle fusion and trafficking as unitary events. More intriguingly, the quantum dot can distinguish different modes of vesicle turnover with previously unmatched clarity. Using this novel approach, we have revealed the kinetics of vesicle recycling and, more importantly, the heterogeneity of turnover routes among different pools of synaptic vesicles. Furthermore, we have demonstrated the close coordination of vesicle trafficking with neuronal activity, a functional demonstration of presynaptic modulation in network strength. We foresee a further empowerment of neuroscientists through the unique and versatile features of these shining nanocrystal particles.
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